Canadian Macromolecular Crystallography Facility

Beamline 08B1-1


Beamline 08B1-1 is ideal for:

  • SAD/MAD experiments, including S-SAD
  • experiments requiring Kappa geometry
  • EXAFS & XANES on crystals
  • experiments using a controlled humidity environment
  • routine screening & data collection
  • remote data collection
  • crystals of peptides or other small biological molecules

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Beamline Overview

Designed for complementary performance to the 08ID-1 beamline, the 08B1-1 beamline is used for screening crystals, high-throughput crystallographic analysis of well-ordered crystals and other more specialized analyses. The design incorporates white beam slits (WBS), a vertical collimating mirror (VCM), a double crystal monochromator (DCM) with indirectly water-cooled first crystal, and a toroidal focusing mirror. The endstation includes a versatile ACCEL MD2 microdiffractometer and MarMosaic mx300 CCD X-ray detector. Like on the 08ID-1 beamline, beamline controls are operated through MxDC.

The 08B1-1 bending magnet produces a lower photon flux than the 08ID-1 insertion device which means that exposure times for crystals on the 08B1-1 beamline are longer than on the 08ID-1 beamline; however, the energy range of this very stable beamline extends to 4.0 keV, where  anomalous and dispersive signals from naturally-occurring sulfur atoms and other lighter elements are higher.

The beamline is additionally equipped with Vortex ME4 & Röntec XFlash 1001 detectors to perform X-ray spectroscopy for MAD/SAD, X-ray Absorption Near Edge Structure (XANES) and Extended X-ray Absorption Fine Structure analyses on crystals. Additionally, the endstation is equipped with an automounter robot allowing for remote experiments as well as onsite work. The goniometer is equipped with a mini-kappa device. An HC1 humidity control device is also available for use on this beamline.

Hardware Details

Source Bending magnet
Current 250 mA
Average Beam Lifetime 12 h
  • Bend radius: 7.14287 m
  • Horizontal fan available: 6 mrad
Minimum Energy 4.0 keV (3.1 Å)
Maximum Energy 18 keV (0.69 Å)
Energy Bandwidth (ΔE/E) 1.4 x 10-4
Flux on Sample @ 12 keV (250 mA) >1E11 ph/s
1 mrad horizontal fan
Focal Size (FWHM) @ 12 keV (H x V) 230 µm x 195 µm
Flux Density @ 12 keV > 1E12 ph/s/mm²
1 mrad fan
Beam Divergence at Sample (H x V) @ 12 keV 1.8 mrad x 0.3 mrad
Available aperture sizes 20, 50, 100, 150, 200 microns
  • Collimating Mirror with two stripes (Si, Rh/Pt)
  • Toroidal Focusing Mirror (Rh/Pt)
Horizontal Demagnification (1/Mx) 2.01
Vertical Demagnification (1/My) 1.37
Crystal Type Si(111)
Monochromator KOHZU double crystal monochromator (DCM), featuring indirectly water-cooled first crystal and flat, long second crystal
Additional Instrumentation Bruker Xflash, Vortex ME4, HC1 humidity control device
Pin Length 18 mm Hampton Cryopin
Cryo Capability Oxford Instruments Cryojet / Cryojet 5 (85 - 500K)
Automounter Stanford Automated Mounting system (SAM) with Unipucks, SSRL cassettes
Goniometer Model Integrated in MD2 microdiffractometer
Maximum Rotation Speed (Deg/s) 120
Positioning Error (mDeg) 2
Sphere of Confusion (microns) 2
Visualization System
Type On-Axis Visualization (OAV)
Orientation Along beam axis
Maximum zoom 12x
Maximum Field of View 1.8 x 1.5 mm
Minimum Field of View 0.18 x 0.12 mm
Crystal-Detector Distance 115-700 mm
Current Detector Model Rayonix MX300HE CCD X-ray detector
Type 16 CCDs, 16 tiled fiber-optic tapers
X-ray Sensitive Surface 300 x 300 mm (90,000 mm²)
Head Dimensions (H x W x D) 58 x 42 x 43 cm
2Θ Range 20° - not currently in use
Resolution 4096 x 4096 (73 µm)
Detector History
  • August 25, 2015 - present: Rayonix MX300HE detector
  • July 22, 2015 - August 20, 2015: Rayonix MX225 detector
  • Before July 22, 2015: Rayonix MX300HE detector


Component Distance from Source
WBS (White Beam Slits) 11.5 m
Mirror M1 (Vertical Collimating) 13.0 m
DCM (Double Crystal Monochromator) 17.1 m
Mirror M2 (Toroidal) 19.1 m
Sample 28.6 m