Canadian Macromolecular Crystallography Facility


Participants in the CLS Mx Data Collection School are expected to have a basic grounding in crystallography.  This includes familiarity with the following subjects:

  1. Protein Crystals
    • Definition of a crystal
    • Judging crystal quality
    • Matthews coefficient, water content
    • Size, integrity and mosaicity
    • Cryoprotection and preparing crystals for data collection
    • Elements of symmetry in protein crystals, point groups, Bravais lattices, Laue groups and space groups
  2. Scattering of X-rays by crystals and Diffraction
    • Interaction of X-rays with matter
    • Interference of scattered waves, structure factors
    • Diffraction conditions, Laue conditions, Bragg's Law
    • Reciprocal lattice and Ewald construction, Miller indices, Fractional coordinates
    • Calculation of electron density from structure factor
    • Crystallographic "phase problem"
  3. Unix-like operating systems

For review, the following sources can be consulted:

  • "Crystallography Made Crystal Clear" by Gale Rhodes
  • "Outline of Crystallography for Biologists" by David Blow
  • CSIC Crystallography