CANADIAN MACROMOLECULAR CRYSTALLOGRAPHY FACILITY

CMCF-BM

Canadian Macromolecular Crystallography Facility - Bending Magnet

  Maintenance & Development

Designed for complementary performance to the CMCF-ID beamline, the CMCF-BM (08B1) beamline is used for screening crystals, high-throughput crystallographic analysis of well-ordered crystals and other more specialized analyses. The design incorporates white beam slits (WBS), a vertical collimating mirror (VCM), a double-crystal monochromator (DCM) / double-multilayer monochromator (DMM), and a toroidal focusing mirror. The endstation includes a versatile ACCEL MD2 microdiffractometer and Pilatus3 S 6M X-ray detector. Like on the 08ID-1 beamline, data collection is controlled through MxDC.

The CMCF-BM bending magnet produces a lower photon flux than the CMCF-ID insertion device in the DCM mode, which means that exposure times are typically longer than on CMCF-ID. The recent incorporation of a double-multilayer monochromator allows high-flux at fixed energy (8.157 keV), the flux in this mode being comparable to that of the current insertion device beamline setup. More information about the upgrade project is available here.

CMCF-BM is additionally equipped with Vortex ME4 & Röntec XFlash 1001 detectors to perform X-ray spectroscopy for MAD/SAD experiments, metal identification, X-ray Absorption Near Edge Structure (XANES) and Extended X-ray Absorption Fine Structure (EXAFS) analyses on crystals. A SAM automounter allows for remote experiments. The goniometer is equipped with a mini-kappa device. An HC1 humidity control device is also available for use on the beamline.

Schematic

Layout
Component Distance from Source
WBS (White Beam Slits) 11.5 m
Mirror M1 (Vertical Collimating) 13.0 m
DCM (Double Crystal Monochromator) 17.1 m
Mirror M2 (Toroidal) 19.1 m
Sample 28.6 m

Beamline Specifications

Source
Typical Ring Current 150-220 mA (present maximum after injection)
Typical Beam Lifetime 20 hr; injections every 8 hr
Bending Magnet
Bend Radius 7.14287 m
Horizontal fan available 6 mrad

Beamline Hardware

Optics
Mirrors Collimating Mirror with two stripes (Si, Rh/Pt)
Toroidal Focusing Mirror (Rh/Pt)
Horizontal Demagnification (1/Mx) 2.01(19.1:9.5)
Vertical Demagnification (1/My) 1.37(13:9.5)
Crystal Type Si(111)
Monochromator KOHZU double crystal monochromator (DCM), featuring indirectly water-cooled first crystal and flat, long second crystal
Endstation
Additional Instrumentation Bruker Xflash, Vortex ME4
HC1 humidity control device
Pin Length 18 mm Hampton Cryopin
Cryo Capability Oxford Instruments Cryojet / Cryojet 5 (85 - 500K)
Automounter Stanford Automated Mounting system (SAM) with Unipucks, SSRL cassettes
Goniometer
Goniometer Model Integrated in MD2 microdiffractometer
Maximum Rotation Speed (Deg/s) 120
Positioning Error (mDeg) 2
Sphere of Confusion (microns) 2
Visualization System
Type On-Axis Visualization (OAV)
Orientation Along beam axis
Maximum zoom 12x
Maximum Field of View 1.8 x 1.5 mm
Minimum Field of View 0.18 x 0.12 mm
Detector
Crystal-Detector Distance 115-700 mm
Current Detector Model Pilatus3 S 6M
Framing Rate 25 Hz
X-ray Sensitive Surface 423.6 x 434.6 mm (184,097 mm²)
Head Dimensions (H x W x D) 59.0 x 60.3 x 45.5 cm
2Θ Range 20° - not currently in use
Resolution 2463 x 2527
Detector History June 2020 - Present: Pilatus3 S 6M
August 25, 2015 - May 2020: Rayonix MX300HE
July 22, 2015 - August 20, 2015: Rayonix MX225
Before July 22, 2015: Rayonix MX300HE

Techniques

Single Crystal X-ray Diffraction including:

  • MAD, SAD, S-SAD experiments
  • XRF, EXAFS & XANES on crystals
  • Humidity Controlled experiments
  • Routine screening & data collection

Powder Diffraction

Small Molecule Crystallography