CANADIAN MACROMOLECULAR CRYSTALLOGRAPHY FACILITY

CMCF-ID

Canadian Macromolecular Crystallography Facility - Insertion Device

  Upgrade In Progress

The scientific goal of the CMCF-ID (08ID) beamline is to operate a protein crystallography beamline suitable for studying small crystals and crystals with large unit cells. The overall design of the beamline consists of white beam slits (WBS), a double crystal monochromator (DCM) with an indirectly cryo-cooled first crystal and sagittally focusing second crystal, and vertical focusing mirror (VFM). The beamline is completed with an innovative endstation and a Pilatus3 S 6M X-ray detector. The beamline controls are based on EPICS and are complemented with MxDC, a user-friendly interface developed in-house.

The source of X-rays on CMCF-ID is a small-gap undulator inserted into the storage ring (and is hence termed an "insertion device"). As its name suggests, the undulator produces a periodic magnetic field that causes the path of the orbiting electrons to undulate, resulting in an emitted X-ray beam of higher spectral brilliance than could be produced by the simpler path taken by the electrons through a bending magnet.

The beamline is equipped with a Röntec XFlash 1001 to perform X-ray spectroscopy for MAD/SAD experiments and metal identification. An ISARA automounter allows remote experiments.

The CMCF-ID beamline is currently undergoing a major upgrade. A description of the upgrade, and progress, can be found on the Upgrade Project page. This page will be replaced soon, with specifications for the upgraded beamline.

Schematic

Layout
Component Distance from Source
WBS (White Beam Slits) 41.3 m (Horizontal Slits) 41.5 m (Vertical Slits)
DCM (Double Crystal Monochromator) 43.5 m
VFM (Vertically Focusing Mirror) 46.0 m
Sample 49.9 m

Beamline Specifications

Source
Typical Ring Current 150-220 mA (present maximum after injection)
Typical Lifetime (1/e) 20 hr; injections every 8 hr
Insertion Device 20 mm asymmetric hybrid small-gap in-vacuum undulator (SGU)
Insertion Device (SGU) Details
Gap Range 5mm - 35mm
Number of Poles (Full Size / Total) 157 / 159
Total Length of Magnet Assemblies 1607mm
Permanent Magnet Blocks Sm2Co17
Minimum Remanance 1.03T
Minimum Intrinsic Coercivity at 20°C 20kOe
Temperature Coefficient, Intrinsic Coercivity -0.19 %/°C
Poles Vanadium Permendur
Saturation Flux Density 2.4T
Total Power 4.0kW
SGU Properties at Minimum Gap
Peak Flux Density 1.066 T
Effective Flux Density 0.981 T
Effective k 1.83
Higher Order Contributions 8.4%
Photon Energy, n = 1 1.49 keV
Maximum Magnetic Load 9.5 kN
Beam
Minimum Energy 6.0 keV (2.1 Å)
Maximum Energy 18 keV (0.69 Å)
Energy Bandwidth (ΔE/E) 1.5 x 10-4
Flux on Sample @ 12 keV (250 mA): Focus 5E12 ph/s
Flux on Sample @ 12 keV (250 mA): 100 µm 2E12
Flux on Sample @ 12 keV (250 mA): 50 µm, current beam size 1E12
Flux on Sample @ 12 keV (250 mA): 20 µm 7E11
Focal Size (FWHM) @ 12 keV (H x V) 150 µm x 50 µm
Flux Density @ 12 keV > 1E14 ph/s/mm²
Beam Divergence at Sample (H x V) @ 12 keV 0.9 mrad x 0.2 mrad

Beamline Hardware

Optics
Monochromator ACCEL/BRUKER double crystal monochromator (DCM, Si-111), featuring indirectly cryo-cooled first crystal and sagittally focusing second crystal
Vertical Focusing Mirror ultra-low expansion (ULE) titanium siliicate flat mirror with: Pt, Uncoated, & Pd strips
Horizontal Demagnification (1/Mx) 6.8
Vertical Demagnification (1/My) 12.0
Endstation
Additional Instrumentation Rontec Xflash 1001 fluorescence detector, HC1 humidity control device
Pin Length 18 mm Hampton Cryopin
Cryo Capability Oxford Instruments Cryojet, 90-300K
Automounter ISARA (single gripper) supporting Uni-Pucks, 464 pin capacity, approx 20 s duty-cycle
Goniometer
Goniometer Huber EZ0675 XY air-bearing
Maximum Rotation Speed (deg/s) 180
Sphere of Confusion (microns) 1
Visualization System
Type On-Axis Visualization (OAV)
Orientation Along beam axis
Maximum zoom 5x
Maximum Field of View 2.9 x 2.2 mm
Minimum Field of View 0.6 x 0.4 mm
Detector
Current Detector Upgrading to Eiger X 9M
Crystal-Detector Distance
Readout Time Continuous readout, 3.0 us dead time, duty cycle >99%
X-ray Sensitive Surface (W x H) 245.2 x 233.2 mm (57,180.64 mm²)
Head Dimensions (W x H x D) 34.0 x 37.0 x 50.0 cm
2Θ Range 20° - not currently in use
Resolution 3,110 x 3,269
Detector History May 2017 - May 2020: Pilatus3 S 6M
April 2010 - February 2017: Rayonix MX300 CCD
Before April 2010: Rayonix MX225 CCD

Techniques

Single crystal X-ray diffraction including:

  • Routine data collection
  • MAD, SAD, S-SAD experiments
  • XRF on crystals