CANADIAN MACROMOLECULAR CRYSTALLOGRAPHY FACILITY

CMCF-ID

Canadian Macromolecular Crystallography Facility - Insertion Device

  Upgrade In Progress

The scientific goal of the CMCF-ID (08ID) beamline is to operate a protein crystallography beamline suitable for studying small crystals and crystals with large unit cells. The overall design of the beamline consists of white beam slits (WBS), a double crystal monochromator (DCM) with an indirectly cryo-cooled first crystal and sagittally focusing second crystal, and vertical focusing mirror (VFM). The beamline is completed with an innovative endstation and a Pilatus3 S 6M X-ray detector. The beamline controls are based on EPICS and are complemented with MxDC, a user-friendly interface developed in-house.

The source of X-rays on CMCF-ID is a small-gap undulator inserted into the storage ring (and is hence termed an "insertion device"). As its name suggests, the undulator produces a periodic magnetic field that causes the path of the orbiting electrons to undulate, resulting in an emitted X-ray beam of higher spectral brilliance than could be produced by the simpler path taken by the electrons through a bending magnet.

The beamline is equipped with a Röntec XFlash 1001 to perform X-ray spectroscopy for MAD/SAD experiments and metal identification. An ISARA automounter allows remote experiments.

The CMCF-ID beamline is currently undergoing a major upgrade. A description of the upgrade, and progress, can be found on the Upgrade Project page.

Schematic

Layout
Component Distance from Source
WBS (White Beam Slits) 41.3 m (Horizontal Slits) 41.5 m (Vertical Slits)
DCM (Double Crystal Monochromator) 43.5 m
VFM (Vertically Focusing Mirror) 46.0 m
Sample 49.9 m

Beamline Specifications

Source
Typical Ring Current 150-220 mA (present maximum after injection)
Typical Lifetime (1/e) 20 hr; injections every 8 hr
Insertion Device 20 mm asymmetric hybrid small-gap in-vacuum undulator (SGU)
Insertion Device (SGU) Details
Gap Range 5mm - 35mm
Number of Poles (Full Size / Total) 157 / 159
Total Length of Magnet Assemblies 1607mm
Permanent Magnet Blocks Sm2Co17
Minimum Remanance 1.03T
Minimum Intrinsic Coercivity at 20°C 20kOe
Temperature Coefficient, Intrinsic Coercivity -0.19 %/°C
Poles Vanadium Permendur
Saturation Flux Density 2.4T
Total Power 4.0kW
SGU Properties at Minimum Gap
Peak Flux Density 1.066 T
Effective Flux Density 0.981 T
Effective k 1.83
Higher Order Contributions 8.4%
Photon Energy, n = 1 1.49 keV
Maximum Magnetic Load 9.5 kN
Beam
Minimum Energy 6.0 keV (2.1 Å)
Maximum Energy 18 keV (0.69 Å)
Energy Bandwidth (ΔE/E) 1.5 x 10-4
Flux on Sample @ 12 keV (250 mA): Focus 5E12 ph/s
Flux on Sample @ 12 keV (250 mA): 100 µm 2E12
Flux on Sample @ 12 keV (250 mA): 50 µm, current beam size 1E12
Flux on Sample @ 12 keV (250 mA): 20 µm 7E11
Focal Size (FWHM) @ 12 keV (H x V) 150 µm x 50 µm
Flux Density @ 12 keV > 1E14 ph/s/mm²
Beam Divergence at Sample (H x V) @ 12 keV 0.9 mrad x 0.2 mrad

Beamline Hardware

Optics
Monochromator ACCEL/BRUKER double crystal monochromator (DCM, Si-111), featuring indirectly cryo-cooled first crystal and sagittally focusing second crystal
Vertical Focusing Mirror ultra-low expansion (ULE) titanium siliicate flat mirror with: Pt, Uncoated, & Pd strips
Horizontal Demagnification (1/Mx) 6.8
Vertical Demagnification (1/My) 12.0
Endstation
Additional Instrumentation Rontec Xflash 1001 fluorescence detector, HC1 humidity control device
Pin Length 18 mm Hampton Cryopin
Cryo Capability Oxford Instruments Cryojet, 90-300K
Automounter ISARA (single gripper) supporting Uni-Pucks, 464 pin capacity, approx 20 s duty-cycle
Goniometer
Goniometer Huber EZ0675 XY air-bearing
Maximum Rotation Speed (deg/s) 180
Sphere of Confusion (microns) 1
Visualization System
Type On-Axis Visualization (OAV)
Orientation Along beam axis
Maximum zoom 5x
Maximum Field of View 2.9 x 2.2 mm
Minimum Field of View 0.6 x 0.4 mm
Detector
Current Detector Upgrading to Eiger X 9M
Crystal-Detector Distance
Readout Time Continuous readout, 3.0 us dead time, duty cycle >99%
X-ray Sensitive Surface (W x H) 245.2 x 233.2 mm (57,180.64 mm²)
Head Dimensions (W x H x D) 34.0 x 37.0 x 50.0 cm
2Θ Range 20° - not currently in use
Resolution 3,110 x 3,269
Detector History May 2017 - May 2020: Pilatus3 S 6M
April 2010 - February 2017: Rayonix MX300 CCD
Before April 2010: Rayonix MX225 CCD

Techniques

Single crystal X-ray diffraction including:

  • Routine data collection
  • MAD, SAD, S-SAD experiments
  • XRF on crystals