Canadian Macromolecular Crystallography Facility - Insertion Device
The scientific goal of the 08ID-1 beamline is to operate a protein crystallography beamline suitable for studying small crystals and crystals with large unit cells. The overall design of the beamline consists of white beam slits (WBS), a double crystal monochromator (DCM) with an indirectly cryo-cooled first crystal and sagittally focusing second crystal, and vertical focusing mirror (VFM). The beamline is completed with an innovative endstation and a Pilatus3 S 6M X-ray detector. The beamline controls are based on EPICS and are complemented with MxDC, a user-friendly interface developed in-house.
The source of X-rays on 08ID-1 is a small-gap undulator inserted into the storage ring (and is hence termed an "insertion device"). As its name suggests, the undulator produces a periodic magnetic field that causes the path of the orbiting electrons to undulate, resulting in an emitted X-ray beam of higher spectral brilliance than could be produced by the simpler path taken by the electrons through a bending magnet.
The beamline is equipped with a Röntec XFlash 1001 to perform X-ray spectroscopy for MAD/SAD experiments and metal identification. An ISARA automounter allows remote experiments.
The CMCF-ID beamline is currently undergoing a major upgrade. A description of the upgrade, and progress, can be found on the Upgrade Project page.
Schematic
| Component | Distance from Source |
|---|---|
| WBS (White Beam Slits) | 41.3 m (Horizontal Slits) 41.5 m (Vertical Slits) |
| DCM (Double Crystal Monochromator) | 43.5 m |
| VFM (Vertically Focusing Mirror) | 46.0 m |
| Sample | 49.9 m |
Beamline Specifications
| Typical Ring Current | 150-220 mA (present maximum after injection) |
|---|---|
| Typical Lifetime (1/e) | 20 hr; injections every 8 hr |
| Insertion Device | 20 mm asymmetric hybrid small-gap in-vacuum undulator (SGU) |
| Gap Range | 5mm - 35mm |
|---|---|
| Number of Poles (Full Size / Total) | 157 / 159 |
| Total Length of Magnet Assemblies | 1607mm |
| Permanent Magnet Blocks | Sm2Co17 |
| Minimum Remanance | 1.03T |
| Minimum Intrinsic Coercivity at 20°C | 20kOe |
| Temperature Coefficient, Intrinsic Coercivity | -0.19 %/°C |
| Poles | Vanadium Permendur |
| Saturation Flux Density | 2.4T |
| Total Power | 4.0kW |
| Peak Flux Density | 1.066 T |
|---|---|
| Effective Flux Density | 0.981 T |
| Effective k | 1.83 |
| Higher Order Contributions | 8.4% |
| Photon Energy, n = 1 | 1.49 keV |
| Maximum Magnetic Load | 9.5 kN |
| Minimum Energy | 6.0 keV (2.1 Å) |
|---|---|
| Maximum Energy | 18 keV (0.69 Å) |
| Energy Bandwidth (ΔE/E) | 1.5 x 10-4 |
| Flux on Sample @ 12 keV (250 mA): Focus | 5E12 ph/s |
| Flux on Sample @ 12 keV (250 mA): 100 µm | 2E12 |
| Flux on Sample @ 12 keV (250 mA): 50 µm, current beam size | 1E12 |
| Flux on Sample @ 12 keV (250 mA): 20 µm | 7E11 |
| Focal Size (FWHM) @ 12 keV (H x V) | 150 µm x 50 µm |
| Flux Density @ 12 keV | > 1E14 ph/s/mm² |
| Beam Divergence at Sample (H x V) @ 12 keV | 0.9 mrad x 0.2 mrad |
Beamline Hardware
| Monochromator | ACCEL/BRUKER double crystal monochromator (DCM, Si-111), featuring indirectly cryo-cooled first crystal and sagittally focusing second crystal |
|---|---|
| Vertical Focusing Mirror | ultra-low expansion (ULE) titanium siliicate flat mirror with: Pt, Uncoated, & Pd strips |
| Horizontal Demagnification (1/Mx) | 6.8 |
| Vertical Demagnification (1/My) | 12.0 |
| Additional Instrumentation | Rontec Xflash 1001 fluorescence detector, HC1 humidity control device |
|---|---|
| Pin Length | 18 mm Hampton Cryopin |
| Cryo Capability | Oxford Instruments Cryojet, 90-300K |
| Automounter | ISARA (single gripper) supporting Uni-Pucks, 464 pin capacity, approx 20 s duty-cycle |
| Goniometer | Huber EZ0675 XY air-bearing |
|---|---|
| Maximum Rotation Speed (deg/s) | 180 |
| Sphere of Confusion (microns) | 1 |
| Type | On-Axis Visualization (OAV) |
|---|---|
| Orientation | Along beam axis |
| Maximum zoom | 5x |
| Maximum Field of View | 2.9 x 2.2 mm |
| Minimum Field of View | 0.6 x 0.4 mm |
| Current Detector | Upgrading to Eiger X 9M |
|---|---|
| Crystal-Detector Distance | |
| Readout Time | Continuous readout, 3.0 us dead time, duty cycle >99% |
| X-ray Sensitive Surface (W x H) | 245.2 x 233.2 mm (57,180.64 mm²) |
| Head Dimensions (W x H x D) | 34.0 x 37.0 x 50.0 cm |
| 2Θ Range | 20° - not currently in use |
| Resolution | 3,110 x 3,269 |
| Detector History |
May 2017 - May 2020: Pilatus3 S 6M April 2010 - February 2017: Rayonix MX300 CCD Before April 2010: Rayonix MX225 CCD |