CANADIAN MACROMOLECULAR CRYSTALLOGRAPHY FACILITY

Upgrade Project

1: Toward Cutting-Edge Science

Establishing advanced micro-beam capability for macromolecular crystallography at the CLS

This is a CFI project led by the Beamline Advisory Team leader Mirek Cygler (U of Saskatchewan), in collaboration with other Beamline Advisory Team members and principal users representing institutes from accross Canada including: Bhushan Nagar (McGill U), Zongchao Jia (Queen's U), Filip Van Petegem (U of British Columbia), Mark Glover (U of Alberta), Kenneth Ng (U of Calgary), Brian Mark (U of Manitoba), Emil Pai (U of Toronto), Alisdair Boraston (U of Victoria) and Alba Guarné (McMaster U).

The project secured funding in 2015 (see announcement here), with the goal of upgrading the CMCF-ID beam line; increasing the flux, reducing the beam focus size to micro-beam, improving the stability of the end-station and improving the data acquisition throughput using a faster detector and automounter. To accomplish these goals, the undulator is being replaced with a longer one, the optical layout is being changed, and the end station being rebuilt.

The new optics, which includes a new DCM/DMM monochromator and three new mirrors, will enable the beamline to achieve a focus spot size of 5 × 30 µm with a photon-flux of about 1 × 1013 ph/s in a 5 µm focus spot, or up to 1 × 1014 ph/s in pink-beam (1% ΔE/E)  mode. Additionally, the endstation will be replaced with an MD2S microdiffractometer, while the previous Pilatus 6M detector (now on CMCF-BM) will be replaced with an Eiger 9M detector. The recently-upgraded ISARA automounter will continue to be a key component of the upgraded beamline.

In April 2019, the preliminary design report was completed. All major components including the insertion device (IVU), DCM/DMM, mirror systems, microdiffractometer, and Eiger detector have been purchased, with delivery times ranging from July 2019 to Fall 2020.

The CMCF-ID beamline was shut down for upgrades during the spring 2020 facility shutdown period. The new insertion device has been installed in the ring, and the new beamline components are currently being installed. We expect the beamline to be returned to service during Summer 2021.  To alleviate the loss of beam time to users during this period, we are accommodating most experiments on the CMCF-BM beamline, which has undergone a successful minor upgrade for higher flux and increased throughput.

2: CMCF-BM High-Flux Upgrade

Multi-layers for higher flux & a faster robot

This small project to upgrade the CMCF-BM Si-111 double-crystal monochromater (DCM) was initiated in January 2019 and completed in August 2019. The goal was to add a fixed-energy (8.157 keV), high-flux mode to the beamline by incorporating a double Multi-Layer monochromator (DMM) inside the same Si-111 DCM chamber. The upgrade was successful and resulted in 2x more flux for the Si-111 mode, and 40x more flux in the Multi-Layer mode. We have collected test datasets to confirm the performance.

Going forward, the CMCF-BM monochromator will operate in two modes. The Multi-Layer (DMM) mode will be a pink-beam (0.45% ΔE/E), delivering the higher flux. Experiments requiring a higher energy resolution will continue to be supported with the traditional Si-111 DCM mode. At this time, switching between the high-flux mode (Multi-Layer, or DMM), and the traditional mode (DCM) is performed by staff (a process which normally takes approximately 5-10 minutes). Please confirm with your local contact as to which mode will be most appropriate for your work, or to change modes.

To complement the higher flux, the SAM Automounter software was also updated to support a faster throughput, with a duty cycle of about 25 seconds (previously 2.5 minutes). Additionally, the Pilatus 6M detector, previously on CMCF-ID, has been permanently moved to CMCF-BM. With these changes, CMCF-BM users are experiencing a significant increase in throughput.

Progress

CMCF-ID Undulator
The first major component of the CMCF-ID upgrade project arrived in August 2019. Pictured above is the new in-vacuum undulator being delivered to the CLS. Travelling first by ship and arriving in Saskatoon by train, the new undulator weighs approximately 12 tons and will be installed in the storage ring during the spring 2020 shutdown.

Eiger Detector
Eiger X 9M detector, delivered November 2019. It is physically smaller than the Pilatus 6M, but 10x faster and with higher pixel density. It will be installed at CMCF-ID and the Pilatus 6M currently on the beamline will be moved to CMCF-BM during the spring 2020 maintenance shutdown.

MD2S
MD2S Micro Diffractometer delivered in November 2019, destined to be installed at CMCF-ID during the upgrade.

CMCF-ID Monochromator.png
Delivery of the new CMCF-ID monochromator, in October 2020.

CMCF Upgrade Timeline