CANADIAN MACROMOLECULAR CRYSTALLOGRAPHY FACILITY

Upgrade Project

1: Toward Cutting-Edge Science

Establishing advanced micro-beam capability for macromolecular crystallography at the CLS

This is a CFI project led by the Beamline Advisory Team leader Mirek Cygler (U of Saskatchewan), in collaboration with other Beamline Advisory Team members and principal users representing institutes from accross Canada including: Bhushan Nagar (McGill U), Zongchao Jia (Queen's U), Filip Van Petegem (U of British Columbia), Mark Glover (U of Alberta), Kenneth Ng (U of Calgary), Brian Mark (U of Manitoba), Emil Pai (U of Toronto), Alisdair Boraston (U of Victoria) and Alba Guarné (McMaster U).

The project secured funding in 2015 (see announcement here), with the goal of upgrading the CMCF-ID beam line,  increasing the flux, reducing the beam focus size to micro-beam, improving the stability of the end-station and improving the data acquisition throughput using a faster detector and automounter.  To accomplish these goals, the undulator will be replaced with a longer one, the optical layout will be changed and the end station will be rebuilt.

The new optics, which includes a new DCM/DMM monochromator and three new mirrors, will enable the beamline to achieve a focus spot size of 5 × 30 µm with a photon-flux of about 1 × 1013 ph/s in a 5 µm focus spot, or up to 1 × 1014 ph/s in pink-beam (1% ΔE/E)  mode. Additionally, the endstation will be replaced with an MD2S microdiffractometer, while the current Pilatus 6M detector will be replaced with an Eiger 9M detector. The recently-upgraded ISARA automounter will continue to be a key component of the upgraded beamline.

As of April 2019, the preliminary design report has been completed, and all major components including the insertion device (IVU), DCM/DMM, mirror systems, microdiffractometer, and Eiger detector have been purchased, with delivery times ranging from July 2019 to May 2020.

We anticipate shutting down the CMCF-ID beamline for upgrades during the spring 2020 facility shutdown period, during which time the new insertion device will be installed, and installation of the new beamline components will begin. Commissioning is expected to last about 6 months, with an expected return to service in January 2021.  To alleviate the loss of beam time to users during this period, we will accommodate most of our users on the CMCF-BM beamline, which is also receiving a minor upgrade.

2: CMCF-BM High-Flux Upgrade

Multi-layers for higher flux & a faster robot

This small project to upgrade the CMCF-BM Si-111 double-crystal monochromater (DCM) was initiated in January 2019 and completed in August 2019. The goal was to add a fixed-energy (8.157 keV), high-flux mode to the beamline by incorporating a double Multi-Layer monochromator (DMM) inside the same Si-111 DCM chamber. The upgrade was successful and resulted in 2x more flux for the Si-111 mode, and 40x more flux in the Multi-Layer mode. We have collected test datasets to confirm the performance.

Going forward, the CMCF-BM monochromator will operate in two modes. The Multi-Layer (DMM) mode will be a pink-beam (0.45% ΔE/E), delivering the higher flux. Experiments requiring a higher energy resolution will continue to be supported with the traditional Si-111 DCM mode. Please inform CMCF staff if you wish to use the high-flux Multi-Layer mode.

To complement the higher flux, the SAM Automounter software has also been updated to support a faster throughput, with a duty cycle of about 25 seconds (previously 2.5 minutes).  

With these changes, we expect CMCF-BM users to experience a significant increase in throughput. Furthermore, when the CMCF-ID beamline is shut down for upgrades (spring 2020), the Pilatus 6M detector will be moved permanently to CMCF-BM, further increasing the throughput.

Progress

CMCF-ID Undulator
The first major component of the CMCF-ID upgrade project arrived in August 2019. Picutured above is the new in-vacuum undulator being delivered to the CLS. Travelling first by ship and arriving in Saskatoon by train, the new undulator weighs approximately 12 tons and will be installed in the storage ring during the spring 2020 shutdown.

Eiger Detector
Eiger X 9M detector, delivered November 2019. It is physically smaller than the Pilatus 6M, but 10x faster and with higher pixel density. It will be installed at CMCF-ID and the Pilatus 6M currently on the beamline will be moved to CMCF-BM during the spring 2020 maintenance shutdown.

MD2S
MD2S Micro Diffractometer delivered in November 2019, destined to be installed at CMCF-ID during the upgrade.

CMCF Upgrade Timeline