Canadian Macromolecular Crystallography Facility
User Guide –
After registering as a CLS User, there are two types of training required to do experiments with a beamline:
- General safety training (if collecting data on-site)
- Beamline Specific Orientation (if collecting data on-site or remotely)
General Safety Training
For those visiting the CLS and wishing to collect data on-site, general safety training is arranged through the User Services Office. After you register as a CLS User, they will inform you which modules you need to complete. The training is usually done online before you arrive, but you may also do it on-site. Once training is complete, pick up your user badge and radiation monitor from the User Services Office (Room 2038). This will allow you access to the experimental floor. Note that the User Services Office is only open Monday to Friday 8:00am to 4:30pm (excluding holidays), and you will need to present government-issued photo ID before a user badge can be issued.
Beamline Specific Orientation (BSO)
A Beamline Specific Orientation (BSO) must be completed for a specific beamline before conducting experiments on it. You will be met by beamline staff at the beginning of your beamtime to complete the necessary BSO. If you need to contact a staff member, please refer to the Schedule and identify the appropriate local contact for that day.
Alternatively, a Remote BSO must be completed before conducting Remote experiments on a beamline. Normally, this training is done through a visit to the CMCF or successful completion of the Mx Data Collection School. However, if you are experienced at collecting remote data at another synchrotron facility, you may complete this training by phone with one of the beamline staff members. Please contact beamline staff for more information.
An overview of some of the general safety items covered in your BSO follows in the sections below. More information about safety in the experimental hutches is found in the Beamline Operations section of the website.
Users are responsible for:
- Understanding and following safety protocols and adhering to all applicable signage.
- Wearing personal protective equipment when working with potentially dangerous substances (for example, please have close-toed shoes, long pants, eye and hand protection when handling liquid nitrogen).
- Posting proper safety protocols and signs required for experiments.
- Ensuring experimenters working at a beamline have completed the Beamline Specific Orientation (BSO).
- Contacting CLS staff or the Floor Coordinator in case of a problem.
- Paying access fee (if applicable) indicated on the invoice provided during permit sign-on. This may be paid to the Floor Coordinator or Reception desk.
- Backing up data and completing on-site data processing before run has ended.
- Cleaning up the beamline and lab areas before run has ended.
- Reporting missing or broken equipment.
- Disposing of food-related garbage in the appropriate garbage bin.
Users should not attempt to:
- Make repairs or align beamline optics
- Remove covers or unplug detectors or other electrical equipment
- Reboot computers (unless instructed to do so).
- Use computer terminals not assigned to users.
- Floor Coordinator (3639) - in most cases this should be your first call
- Beamline Personnel contact information is posted at the beamlines
- Emergency (911)
- University of Saskatchewan Security (9-966-5555)
- CLSI HSE 24/7 On-call (9-227-3113)
- CLSI HSE Normal Business Hours (3663)
Emergency exits are located around the perimeter of the building. The nearest emergency exit for CMCF is via the South-East stairwell (see map). In case of an emergency (i.e. Fire Alarm or other emergency) proceed through the nearest exit to the front lawn area (see Emergency Evacuation).
Fire extinguishers are located at waist height near room and building exits. At the CMCF, there is a fire extinguisher located near the exit inside each hutch, as well as near the neareste emergency exit (see map). The fire extinguishers around CMCF are type BC. If there is a fire that cannot be put out immediately, calmly leave the building by the nearest Emergency Exit, pulling the fire alarm on the way out. Proceed to the front lawn area for further instructions (see Emergency Evacuation).
Each beamline has a Health and Safety Information Centre (see map), where four items are posted:
Permit. All experiments require a valid permit. The permit is organized through the Users Office and HSE. Without a permit, the floor coordinator or HSE personnel can stop work on a beamline.
Material Safety Data Sheets (MSDS). Instructions for access to electronic MSDS are also found here. If you require any assistance please contact the Floor Coordinator (3639).
Contact List. A list of important contacts such as the Floor Coordinator, HSE, Campus Security and other emergency contacts.
Evactuation Protocol. These describe the proceedures during an alarm or other emergency.
A first aid kit is located in the 08ID-1 Control Area, near the Sample Prep Room in the 08B1-1 area (see map) as well as at other places around the CLS for use in minor first aid. Inside each kit is a standard assortment of supplies capable of handling a small incident. Next to the first aid kit at beamline 08B1-1, there is also an eye wash bottle.
If additional supplies are required the first aid closets contain extras of all items included in the first aid kits. All incidents and uses of supplies should be recorded on the log sheets (included in the kits), regardless of severity.
If you need to prepare large volumes of buffers or make use of hazardous chemicals or other hazardous materials, please do not use the CMCF Sample Prep room for this. The Wet Lab must be used for these purposes (see map). A safety shower and eye wash station are located in the Wet Lab.
Please inform staff if you will be making use of the Wet Lab and label all chemicals and workspace appropriately including the beamline where you can be found. You must display your Wet Lab "permit" in one of the blue permit holders while working in the lab. Note that if you wish to use the Wet Lab you must have indicated on your Proposal that you require Wet Lab facilities. You may submit an amendment to your Proposal if you did not do this. You will have to complete a Laboratory Safety training module before making use of the Wet Lab.
When using the Nitrogen Fill Station, please wear suitable clothing (close-toed shoes and long pants) along with safety gloves and face shield or eye protection, which are provided. A lab coat may also be used for additional protection.
CMCF hutches are equipped with oxygen sensors because liquid nitrogen is used inside them which can displace oxygen under certain circumstances. If oxygen levels ever fall below safe levels, blue warning lights will flash both inside and outside the hutch. If this occurs, do not enter the hutch. Inform the Floor Coordinator (3639) or Health and Safety (3663). If you are inside the hutch when this happens, exit the hutch immediately.
When leaving the beamline for more than 30 minutes while an experiment is running, please fill out a pink "Beamline Unattended" form and post it at the Health & Safety Information Centre.
In the event of a fire alarm or when asked to do so by the Floor Coordinator or other Emergency Response Personnel, proceed to the closest safe exit route (at CMCF, this is via the southeast stairwell - see map). Take your jacket and/or personal belongings if safe to do so. Proceed to the designated evacuation gathering area (CLS lawn area at the front of the building) and report to your beamline scientist or User Office personnel. During non-business hours, report to University of Saskatchewan security personnel. During inclement weather, two locations are available: the Peterson Building and the Canadian Wildlife Service.
Map: Google maps Canada.